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Current textSource: eCFR

§ 40.87 What validity tests must laboratories conduct on primary urine specimens?

Current wording, effective June 1, 2023.

eCFR (current) ↗
What changedNov. 13, 2017 wording → June 1, 2023 wording (current)+179 added−282 removedHide

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(ng/mLa). TheYou tablemust follows:
Initialdetermine testthe analyte
Initialcreatinine test cutoff concentration
Confirmatory teston analyte
Confirmatoryeach testprimary cutoffspecimen. concentration
MarijuanaYou metabolites 50must ng/mL THCA
1 15also ng/mL.
Cocainedetermine metabolites 150its ng/mL Benzoylecgonine 100specific ng/mL.
Opiategravity metabolites
Codeine/Morphine
2 2000if ng/mL Codeine 2000you ng/mL.
Morphine 2000find ng/mL.
6-Acetylmorphine 10the ng/mL 6-Acetylmorphine 10creatinine ng/mL.
Phencyclidine 25concentration ng/mL Phencyclidine 25to ng/mL.
Amphetamines
3
AMP/MAMP
4 500be ng/mL Amphetamine 250less ng/mL.
Methamphetamine
5 250than ng/mL.
MDMA
6 50020 ngmg/mL MDMA 250 ng/mLdL.
MDA
7 250 ng/mL.
MDEA
8 250 ng/mL
1 Delta-9-tetrahydrocannabinol-9-carboxylic acid (THCAb).
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3(c) EitherYou amust singleperform initialone test kit or multiplemore initialvalidity testtests kitsfor mayoxidizing beadulterants usedon providedeach theprimary singlespecimen.
(d) testYou kitmust detectsperform eachadditional targetvalidity analytetests independentlyon at the specifiedprimary cutoff.
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5(1) ToAbnormal bephysical reportedcharacteristics;
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6g., Methylenedioxymethamphetaminenon-recovery (MDMA).
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(b3) OnPossible anunidentified initialinterfering drugsubstance test,or youadulterant.
(e) mustIf reportyou adetermine resultthat below the cutoffspecimen concentration as negative. If the result is atinvalid orand aboveHHS theguidelines cutoffdirect concentration, you mustto conductcontact athe confirmation test.
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[65 FR 79526, Dec. 19, 2000, as amended at 7569 FR 4986264867, AugNov. 169, 2010;2004. 77Redesignated and amended at 88 FR 2647327643, May 42, 20122023]

49 CFR § 40.87 — What validity tests must laboratories conduct on primary urine specimens?. Text current through October 22, 2026. This wording effective June 1, 2023 to present. Source: eCFR (current), https://www.ecfr.gov/current/title-49/section-40.87. Retrieved from FMCSR.com, https://fmcsr.com/reg/40.87, on October 26, 2026. Unofficial compilation; verify against the official source.

As a laboratory, when you conduct validity testing under § 40.86, you must conduct it in accordance with the requirements of this section.

(a) You must determine the creatinine concentration on each primary specimen. You must also determine its specific gravity if you find the creatinine concentration to be less than 20 mg/dL.

(b) You must determine the pH of each primary specimen.

(c) You must perform one or more validity tests for oxidizing adulterants on each primary specimen.

(d) You must perform additional validity tests on the primary specimen when the following conditions are observed:

(1) Abnormal physical characteristics;

(2) Reactions or responses characteristic of an adulterant obtained during initial or confirmatory drug tests (e.g., non-recovery of internal standards, unusual response); or

(3) Possible unidentified interfering substance or adulterant.

(e) If you determine that the specimen is invalid and HHS guidelines direct you to contact the MRO, you must contact the MRO and together decide if testing the primary specimen by another HHS certified laboratory would be useful in being able to report a positive or adulterated test result.

[65 FR 79526, Dec. 19, 2000, as amended at 69 FR 64867, Nov. 9, 2004. Redesignated and amended at 88 FR 27643, May 2, 2023]

Amendment history

7 distinct wordings on file since Oct. 1, 2001
  1. June 1, 2023 → presenteCFR, day-precisediff vs. previousviewing
    [65 FR 79526, Dec. 19, 2000, as amended at 69 FR 64867, Nov. 9, 2004. Redesignated and amended at 88 FR 27643, May 2, 2023]
  2. May 2, 2023 → June 1, 2023eCFR, day-precisediff vs. previous
    [65 FR 79526, Dec. 19, 2000, as amended at 75 FR 49862, Aug. 16, 2010; 77 FR 26473, May 4, 2012; 82 FR 52244, Nov. 13, 2017]
  3. Jan. 1, 2018 → May 2, 2023eCFR, day-precisediff vs. previous
    [65 FR 79526, Dec. 19, 2000, as amended at 75 FR 49862, Aug. 16, 2010; 77 FR 26473, May 4, 2012; 82 FR 52244, Nov. 13, 2017]
  4. Nov. 13, 2017 → Jan. 1, 2018eCFR, day-precisediff vs. previous
    [65 FR 79526, Dec. 19, 2000, as amended at 75 FR 49862, Aug. 16, 2010; 77 FR 26473, May 4, 2012]
  5. Oct. 1, 2012 → Nov. 13, 2017annual editions 2012–2016diff vs. previous
    [65 FR 79526, Dec. 19, 2000, as amended at 75 FR 49862, Aug. 16, 2010; 77 FR 26473, May 4, 2012]
  6. Oct. 1, 2010 → Oct. 1, 2012annual editions 2010–2011diff vs. previous
    [65 FR 79526, Dec. 19, 2000, as amended at 75 FR 49862, Aug. 16, 2010]
  7. Oct. 1, 2001 → Oct. 1, 2010annual editions 2001–2009

A new entry appears whenever the regulatory words change between government snapshots. Day-precise from the eCFR (2017 onward); before that, one snapshot per annual CFR edition (revised as of October 1). Bracketed citations are the Federal Register history printed with the section. Set a case date in the header to read any earlier wording.